Middle-Down Analysis of Monoclonal Antibody Middle Using Nano-flow Liquid Chromatography and a Novel Tribrid Orbitrap Mass Spectrometer

Monoclonal antibodies (mAbs) are an increasingly important line of therapeutics for the biopharmaceutical industry. The demand to better understand the biochemical and biophysical properties of mAbs has become critical. Recent developments in high resolution mass spectrometry with multiple dissociation techniques have clearly shown its distinctive power for characterization of intact proteins and in particular its subunits. The mass spectrometry–based study of mAbs in middle-down approach Expanded view of glycoforms at charge +51 was provides a wealth of information to interpret its structural features. This application note describes the use of an Orbitrap Fusion mass analyzer in combination with nano liquid chromatography for characterizing a mAb protein using different dissociation techniques.