ResourceSpectroscopy

Microscopic analysis of the orientation and secondary structure of spider silk using polarized Raman spectroscopy

14 Mar 2022

In this application note, JASCO demonstrates the structural analysis of spider silk proteins by polarized Raman micro-spectroscopy. This promises to be an effective approach for evaluating the orientation and composition of minute regions such as single fibers and multilayer films.

NRS-4100 Raman Micro-spectrometer

JASCO (USA)

For the first time, the NRS-4100 brings together a number of critical elements to make Raman spectroscopy accessible not only to experienced spectroscopists, but also to first-time users. Features Class 1 laser safety Rigid optical bench and laser image Flexible sampling options Powerful 'UserAssist' control for experienced spectroscopists and new users alike 'Simple Search' function Chemical Image identification and functional groups registry

(1)

NRS-7000 Series Raman Spectrometer

JASCO (USA)

The performance and functions expected on a micro-Raman spectrometer are all provided with the NRS-7000 series Raman systems, assuring consistent performance for rapid acquisition of high quality data with automated system control and minimal optical adjustments. For application expansion, an automated multi-grating turret, 2 internally mounted detectors and a maximum of 8 lasers ranging from the UV through the NIR are capable of integration with the instrument system, all optical components are PC controlled for maximum flexibility with minimum user interaction. Features Research-grade model assuring high spectral quality Exceptional wavenumber accuracy with a high-precision rotary-encoder direct drive mechanism Low wavenumber measurement (NRS-5200/7200) Auto-alignment of microscope laser introduction optics and Raman scattering light path Wavenumber calibration using an integrated Ne lamp Unique Dual Spatial Filter (DSF) for higher spatial resolution than conventional confocal optics Patented Spatial Resolution Image (SRI) function for simultaneous observation of sample image, laser spot and aperture image Full range of options including macro-Raman measurement unit and fiber optic probes

(0)

NRS-5000 Series Raman Spectrometer

JASCO (USA)

The performance and functions expected on a micro-Raman spectrometer are all provided with the NRS-5000 series Raman systems, assuring consistent performance for rapid acquisition of high quality data with automated system control and minimal optical adjustments. For application expansion, an automated multi-grating turret, 2 internally mounted detectors and a maximum of 8 lasers ranging from the UV through the NIR are capable of integration with the instrument system, all optical components are PC controlled for maximum flexibility with minimum user interaction. Features Research-grade model assuring high spectral quality Exceptional wavenumber accuracy with a high-precision rotary-encoder direct drive mechanism Low wavenumber measurement (NRS-5200/7200) Auto-alignment of microscope laser introduction optics and Raman scattering light path Wavenumber calibration using an integrated Ne lamp Unique Dual Spatial Filter (DSF) for higher spatial resolution than conventional confocal optics Patented Spatial Resolution Image (SRI) function for simultaneous observation of sample image, laser spot and aperture image Full range of options including macro-Raman measurement unit and fiber optic probes

(0)

Links

Tags

Raman SpectroscopyRaman spectroscopy is used to discern the vibrational and rotational states of molecules and hence the chemical composition of a sample by measuring the inelastic scattering of monochromatic light. Explore a range of Raman spectrometers, including handheld/portable Raman spectrometers for QC/QA labs and in situ spectrometers for processes. Conduct Raman imaging for microanalysis of mixed samples using a Raman microscope. Raman spectrographs are also available. Find the best Raman spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Particle CharacterizationParticle characterization instruments are used to determine particle size distribution, shape, surface area, zeta potential, density and porosity of particles and materials. Multiple tecchniques are available for determining particle size, shape and count including dynamic light scattering (DLS), laser diffraction, electrozone (Coulter technique), imaging particle analysis and single particle optical sensing. Determine the density of your material with a gas pycnometer or examine its surface area and porosity with gas adsorption analyzers and mercury porosimeters. Find the best particle characterization instruments in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Compound IdentificationProtein StructureProtein structure refers to the three-dimensional arrangement of amino acids in proteins, determining their function. Understanding protein structure is key in drug discovery, enzymology, and molecular biology. Explore protein structure analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.MicroanalysisSpectroscopySpectroscopy is a technique that analyzes the interaction of light with matter to study molecular properties, concentrations, and structural information. Widely used in chemical, pharmaceutical, and environmental analysis, spectroscopy offers insights into molecular composition and helps identify unknown compounds. It plays a key role in quality control, research, and diagnostics. Browse our peer-reviewed product directory to compare spectroscopy tools, read reviews, and get prices directly from manufacturers.Microscience