ResourceLife Sciences
MicroRNA Expression Profiling of Individual Rat Hypothalamic Nuclei
MicroRNA Expression Profiling of Individual Rat Hypothalamic Nuclei
2 Aug 2015This application note demonstrates the acquisition of individual miRNA expression profiles from single hypothalamic nuclei provided by cDNA library sequencing using a GAIIX machine. MicroRNAs (miRNAs) finely tune messenger RNA(mRNA) expression. As the brain is a highly heterogeneous tissue, physiologically relevant miRNA expression profiling greatly benefits from sampling brain regions or nuclei.
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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. 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