Microplate Based Pathlength Correction Method for Photometric DNA Quantification Assay

6 Feb 2015

This technical note describes how to use a simple photometric pathlength correction method to calculate nucleic acid concentrations from sample absorbance measured on a microplate with the Thermo Scientific Multiskan GO spectrophotometer.

Thermo Scientific Multiskan GO

Thermo Fisher Scientific

The Thermo Scientific Multiskan GO UV/Vis microplate spectrophotometer offers free wavelength selection for both 96- and 384-well plates and various types of cuvettes. It features a broad wavelength range with UV area and pathlength correction, as well as fast spectral scanning; making it ideal for virtually any photometric research application, including DNA, RNA and protein analysis. The Multiskan GO can be controlled as a stand-alone with straight-forward internal software for quick and simple measurements. And even the most demanding applications can easily be set up using the intuitive Thermo Scientific SkanIt software for PC control. Both the internal and PC software are available in multiple languages including English, French, German, Spanish, Portuguese, Russian, Chinese and Japanese.Key Features: Performs endpoint, kinetic and spectral scanning assays for 96- and 384-well plates, and cuvettes Compatible with Thermo ScientificTM μDropTM Plate – a quick and easy tool for DNA and RNA assays using sample volumes down to 2 μL Fast plate measurements - full spectrum of a sample in less than 10 seconds High quality data guaranteed by extensive self-diagnostics Automation compatible Application Areas: DNA and RNA quantification and purity Protein assays Immunoassays (ELISA) Cell proliferation and cytotoxicity assays Enzyme assays Reporter gene assays

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Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA QuantificationDNA quantification is the process of measuring the amount of DNA in a sample. It is vital in genomics, molecular biology, and biotechnology, helping to ensure accurate experiments and data analysis. Explore DNA quantification tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.
Microplate Based Pathlength Correction Method for Photometric DNA Quantification Assay