Microinjection into the Gonads of <i>C. elegans</i> Using Eppendorf InjectMan® 4

13 Oct 2014

Microinjection is an established method for generating transgenic C. elegans strains for the study of protein domains and RNA or DNA regulatory elements. Microinjection is an established method for generating transgenic C. elegans strains. This application note describes a microinjection procedure using the Eppendorf InjectMan®4 micromanipulator.

InjectMan® 4 Micromanipulator

Eppendorf

The new InjectMan® 4 is ideal for microinjection into adherent cells, smaller organisms and embryos in the early stages of development. The combination of InjectMan 4, FemtoJet® 4i or 4x enables a fast, semi-automatic injection. Furthermore, the InjectMan 4 is the ideal micromanipulator for all complex applications that require a dynamic movement mode and direct control of the injection process via the joystick button. The axial movement ensures the optimal protection of sensitive cells and lowest possible mortality rate. The high advancing speed makes penetration in rigid structures easier than ever. Maximum stability and highest precision ensures accurate and drift-free work. The new user interface with four pre-defined application masks facilitates and eases the individual workflow process (e.g. for adherent cell injection, serial microinjection into fish larvae, etc.). The freely programmable "My app" mask can be optimized for specific individual needs. Features: Maximum stability ensures drift-free work Selection and programming of additional functions (e.g. axial movement, step injection) Ergonomically shaped control panel for fatigue-free work Easy and precise angle adjustment Connection with Eppendorf PiezoXpert for piezo-supported penetration over a pre-defined distance Can be adapted to all conventional microscopes PC interface for remote control Applications: Semi-automatic microinjection into adherent cells Serial microinjection into fish larvae, frog eggs, insect embryos, etc. Injection into C. elegans and other roundworms and flatworms Automatic dispensing of cell suspensions or solutions

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Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RegulatoryPharmaceutical regulations impact on all areas of drug development, manufacture and control and supply. Services are provided from the early stages of drug development and clinical trials through to dossier submission, approval and marketing. Regulatory also includes submission of analytical studies from the pharmaceutical product and safety. Genetic EngineeringGene Regulation
Microinjection into the Gonads of <i>C. elegans</i> Using Eppendorf InjectMan® 4