ResourceLife Sciences
Microinjection into the Gonads of <i>C. elegans</i> Using Eppendorf InjectMan® 4
13 Oct 2014Microinjection is an established method for generating transgenic C. elegans strains for the study of protein domains and RNA or DNA regulatory elements. Microinjection is an established method for generating transgenic C. elegans strains. This application note describes a microinjection procedure using the Eppendorf InjectMan®4 micromanipulator.
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Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RegulatoryPharmaceutical regulations impact on all areas of drug development, manufacture and control and supply. Services are provided from the early stages of drug development and clinical trials through to dossier submission, approval and marketing. Regulatory also includes submission of analytical studies from the pharmaceutical product and safety. Genetic EngineeringGene Regulation
