Microinjection into Plant Cells of Etiolated Seedlings Using the Eppendorf InjectMan® 4

22 Jun 2015

For several model systems microinjection is an established method to introduce DNA into single cells to generate transient and stable transformants. Not only nucleic acids but also proteins and small effectors can be injected also in combination to analyze e.g. cellular signal transduction events. This application note presents the greater efficacy of Eppendorf’s new micromanipulation system in increasing the survival rate of injected plant cells.

InjectMan® 4 Micromanipulator

Eppendorf

The new InjectMan® 4 is ideal for microinjection into adherent cells, smaller organisms and embryos in the early stages of development. The combination of InjectMan 4, FemtoJet® 4i or 4x enables a fast, semi-automatic injection. Furthermore, the InjectMan 4 is the ideal micromanipulator for all complex applications that require a dynamic movement mode and direct control of the injection process via the joystick button. The axial movement ensures the optimal protection of sensitive cells and lowest possible mortality rate. The high advancing speed makes penetration in rigid structures easier than ever. Maximum stability and highest precision ensures accurate and drift-free work. The new user interface with four pre-defined application masks facilitates and eases the individual workflow process (e.g. for adherent cell injection, serial microinjection into fish larvae, etc.). The freely programmable "My app" mask can be optimized for specific individual needs. Features: Maximum stability ensures drift-free work Selection and programming of additional functions (e.g. axial movement, step injection) Ergonomically shaped control panel for fatigue-free work Easy and precise angle adjustment Connection with Eppendorf PiezoXpert for piezo-supported penetration over a pre-defined distance Can be adapted to all conventional microscopes PC interface for remote control Applications: Semi-automatic microinjection into adherent cells Serial microinjection into fish larvae, frog eggs, insect embryos, etc. Injection into C. elegans and other roundworms and flatworms Automatic dispensing of cell suspensions or solutions

(1)

Links

Tags

Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Molecular BiologyMolecular biology is the branch of biology that focuses on the molecular mechanisms that underlie cellular functions. It involves studying DNA, RNA, and proteins to understand gene expression, replication, and regulation. Molecular biology is fundamental to biotechnology, medicine, and genetic research. Explore molecular biology products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Cell ManipulationDNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.Plant SciencePlant science studies the biology of plants, including genetics, physiology, ecology, and biotechnology. Research in this field is essential for improving crop yields, disease resistance, and sustainability. Explore plant science tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.Genetic EngineeringGene Regulation
Microinjection into Plant Cells of Etiolated Seedlings Using the Eppendorf InjectMan® 4