ResourceLife Sciences
Microcalorimetry as a Tool for Structural Biology
8 Sept 2015Crystallization of complexes (protein/protein, protein/nucleic acid, protein/ligand, nucleic acids/nucleic acid, nucleic acid/ligand), even on well-characterized biological systems, are frequently tedious and either time or sample consuming. Isothermal Titration Calorimetry (ITC) is the “gold standard” technique for investigating molecular interactions and in this application note, ITC is shown to be a valuable technique to improve crystallization of complexes. This application note explores the use the Microcal™ iTC200 from Malvern Instruments Ltd.
Links
Tags
AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.CalorimetryCalorimetry is a technique used to determine the heat released or absorbed in a chemical reaction. Calorimeters can be categorized into bomb calorimeters, constant pressure calorimeters, differential scanning calorimeters, isothermal titration calorimeters, X-ray microcalorimeters, heat-loss calorimeters, and high-energy particle calorimeters. The choice of calorimeter depends on the application.TitrationTitration is an analytical technique for determining substance concentration in a solution. For titration consider a manual or automated system, volumetric or coulometric reagent addition and indication by a potentiometer, spectrometer, turbidometer or by the Karl Fischer method. Other features of titration to consider include burettes, reagents, concentration range, reaction time and autosamplers.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.Microflow CalorimeterHigh ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.Protein InteractionsProtein interactions are essential for understanding cellular processes, as proteins work together to carry out biological functions. Studying protein interactions can provide insights into disease mechanisms, drug development, and cell signaling pathways. Explore the best tools for protein interaction research in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Label-Free Analysis