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Micro-Dissection of Sucrose Treated Brain Tissues with Kuiqpick: An Optimized Protocol

NeuroInDx
27 Apr 2014

This application note describes the isolation of single cells, cell clusters, and subanatomical regions in the central nervous system from sucrose treated brain slices. The optimized protocol yields high quality RNA from the micro-dissected material from a coronal section of a mouse brain.

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KUIQPICK

NeuroInDx

KUIQPICK v.1.0 is a capillary-based vacuum-assisted device for tissue microdissection and collection of cells from cell culture dishes. High quality RNA, protein, and DNA are isolated from dissected samples, suitable for genomics and proteomics applications. The advantage of KUIQPICK is its cost effectiveness, flexibility and ease of use compared to existing technologies, such as LCM and FACS.KUIQPICK is an ideal instrument for rapid collection of individual cells from adherent cultures or brain tissue microdissection. Using the X-Y mechanical stage the tissue/cells of interest are located. A single press of a button initiates collection of the tissue area/cells into the disposable capillary unit (DCU). Collected samples are transferred to a test tube for further experimentation.

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NeuroscienceNeuroscience research investigates the neurological mechanisms underlying behavior, neurodegenerative diseases, and other brain conditions. Learn about the innovative technologies for bioimaging, electrophysiology, cell culture, chromatography and other techniques used in this field.

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Micro-Dissection of Sucrose Treated Brain Tissues with Kuiqpick: An Optimized Protocol