Mercury- and Fluorinert® - free Piezo-Assisted Transfer of ES Cells into Mouse Embryos Using the Eppendorf PiezoXpert®

20 Jan 2015

Mutant mouse lines derived from injection of genetically modified embryonic stem cells are important and very powerful tools to analyze gene function. This application note shows that injection of ES cells into eight-cell stage mouse embryos or morulae can be achieved with great success using the Eppendorf PiezoXpert without mercury or Fluorinert filled injection capillaries. Additional advantages of the Eppendorf PiezoXpert are also discussed.

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Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Animal ModelsThe use of non-human animals in experiments or behavorial observations. The research is conducted inside universities, medical schools, pharmaceutical companies, farms, defence establishments, and commercial facilities that provide animal-testing services to industry. It includes pure research such as genetics, developmental biology, behavioral studies, as well as applied research such as pharmaceutical testing in pre-clinical, before human, studies.
Mercury- and Fluorinert® - free Piezo-Assisted Transfer of ES Cells into Mouse Embryos Using the Eppendorf PiezoXpert®