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Measurement of Gold Nanoparticle Solutions on the LAMBDA 265 Personal Array UV/Visible Spectrophotometer

PerkinElmer

Measurement of Gold Nanoparticle Solutions on the LAMBDA 265 Personal Array UV/Visible Spectrophotometer

24 Dec 2015

Gold nanoparticles are of interest in the study of modern materials. They have unique optical, electronic, and thermal properties and are used in a wide range of applications including diagnostics assays, microscopy and electronics. Although of interest in the quest to find novel materials, they have been used since ancient times as a pigment for stained glass. Gold nanoparticle solutions exhibit different optical properties compared with gold in its more familiar “macro” form as they have a reddish color which changes as the particle size increases. The measurement of these optical properties using the LAMBDA™ 265 Personal Array UV/Visible Spectrophotometer is demonstrated in this application note.

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LAMBDA 25, 35, & 45 UV/Vis Spectrophotometers

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PerkinElmer's LAMBDA 25/35/45 UV/Vis systems are easy to operate and deliver trustworthy results with the minimum of operator training. With over 12,000 units installed worldwide, our superior technology and proven build quality has proven itself under the toughest conditions. Our extensive suite of Instrument Performance Verification (IPV) tests confirms high quality performance over the lifetime of the system, and for regulated industries the Enhanced Security (ES) version of UV WinLab integrates seamless 21CFR part 11 technical compliance.LAMBDA 25Choose the LAMBDA 25 for routine UV/Vis applications including liquids analysis, pharmacopeia and regulatory tests. Range: 190 nm - 1100 nm Bandwidth: 1 nm (fixed) LAMBDA 35Choose the LAMBDA 35 for measurements on liquids, solids, pastes and powder samples and regulatory tests requiring variable bandwidths. Range: 190 nm - 1100 nm Bandwidth: 0.5 nm - 4 nm (variable) Integrating sphere option LAMBDA 45Choose the LAMBDA 45 for measurements on turbid and light-scattering liquid samples such as biological solutions and suspensions. Range: 190 nm - 1100 nm Bandwidth: 0.5 nm - 4 nm (variable) Pre-monochromator for reduced stray light

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UV-Visible SpectroscopyUltraviolet-visible (UV-Vis) spectrophotometers are used to measure the interaction of UV and visible light with a sample, including transmission, reflectance & absorbance. The two major instrument classes are single-beam or double-beam spectrophotometers. More specialized equipment includes colorimeters, spectroradiometers and refractometers. Portable and microvolume spectrophotometers are also available. For the modular spectroscopy lab, explore a range of light sources for combination with a spectrograph/spectrometer and optics. Find the best UV-Vis spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NanotechnologyNanotechnology, or nanotech, is an engineering technique using molecular scale functional systems. Applications of nanotechnology include medicine and medical devices, electronics, air and water purification, food science and energy production.Particle CharacterizationParticle characterization instruments are used to determine particle size distribution, shape, surface area, zeta potential, density and porosity of particles and materials. Multiple tecchniques are available for determining particle size, shape and count including dynamic light scattering (DLS), laser diffraction, electrozone (Coulter technique), imaging particle analysis and single particle optical sensing. Determine the density of your material with a gas pycnometer or examine its surface area and porosity with gas adsorption analyzers and mercury porosimeters. Find the best particle characterization instruments in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.Molecular DiagnosticsMolecular diagnostics use an individual’s genetic code and gene expression to diagnose and monitor diseases. The technique is used increasingly in the field of infectious diseases and oncology, as well as areas such as coagulation, HLA typing and pharmacogenomics. Molecular diagnostics plays a pivotal role in personalized medicine.NanoparticlesNanoparticles are between 1-100nm in size. Nanoparticles can be used for a wide variety of applications including biomedical, catalysis and electronics.Optical Properties

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Measurement of Gold Nanoparticle Solutions on the LAMBDA 265 Personal Array UV/Visible Spectrophotometer