Maximizing phosphopeptide recovery in LCMS studies with MaxPeak High Performance Surfaces technology

In this application note, Waters investigates the benefits of MaxPeak HPS technology for the analysis of phosphopeptides. Waters demonstrates how the MaxPeak HPS hardware takes advantage of a hybrid inorganic/organic silica surface layer which is chemically similar to bridged-ethyl hybrid silica. When applied to metallic surfaces wetted during an LC separation, such as the column and LC fluidic path, MaxPeak HPS imparts a resilient barrier but does not participate in separation of sample constituents. Experiments were performed in which the recovery response of numerous phosphopeptides were measured using both conventional and HPS-modified systems.