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Mass-Based Purification of Natural Product Impurities Using an Agilent 1260 Infinity II Preparative LC/MSD System

24 Aug 2020

This Application Note demonstrates the workflow from analytical scouting through scale-up to preparative conditions and purification of a natural product extract by mass-based fraction collection.

Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector

Agilent Technologies

The 1290 Infinity II Preparative Open-Bed Fraction Collector is our most versatile fraction collector, enabling high-capacity fraction collection of purified compounds for semipreparative and preparative-scale LC purification. This module is ideally suited to open-access environments, provides all standard trigger modes for purification workflows, and is optimized for flow rates up to 200 mL/min.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Mass-Based Purification of Natural Product Impurities Using an Agilent 1260 Infinity II Preparative LC/MSD System