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MALDI-TOF MS for species identification in single laboratories or laboratory networks

Bruker Microbiology & Infection Diagnostics
20 Feb 2024

In this application note, Bruker discusses the central role of MALDI-TOF MS in food microbiology and veterinary diagnostics. The note also outlines guidelines for method validations, emphasizing three categories: non-directed identification checks, targeted identification validations, and confirmatory method validations, each tailored to different identification goals.

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MALDI Biotyper® sirius System

Bruker Microbiology & Infection Diagnostics

The MALDI Biotyper® employs MALDI-TOF mass spectrometry for microbial identification, facilitating unbiased identification of microorganisms within a few minutes down to the species level, starting from culture. It boasts various features, such as ease of use, rapidity, robustness, high throughput, cost-effectiveness, and efficiency, making it a must-have for microbiology laboratories.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microbiology ConsumablesFood SafetyFood safety describes the prevention of food-borne illnesses. Contamination with a variety of pathogens can be prevented with adequate temperature control, hygiene and labeling. Food samples can be tested using GC/MS, HPLC, SPE, and qPCR to ensure their adherence to regulations.Food ContaminationFood contamination refers to the presence of harmful substances or pathogens in food, posing risks to human health. Contaminants can be biological, chemical, or physical, and their detection is crucial for ensuring food safety. Advanced testing methods like PCR, chromatography, and spectroscopy are used to identify contaminants in food products. Browse our peer-reviewed product directory to find the best solutions for food contamination detection, compare products, check reviews, and get pricing directly from manufacturers.

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MALDI-TOF MS for species identification in single laboratories or laboratory networks