MAGPIX® Bead Loading Optimization

18 Sept 2014

In a Luminex flow cytometer beads are injected single file through a cuvette and are counted and classified as they pass by a laser. When the bead count for all regions reaches the desired value, the system stops acquisition and moves on to the next well. In the MAGPIX system the entire Sample Load Volume is injected into a chamber where the beads are imaged. The image is then processed by the software and the data is presented to the user. This technical note presents a method for optimizing MAGPIX® bead loading.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Magnetic Beads