ResourceSeparations
mAb titer determination in 60 seconds using the Agilent Bio‑Monolith rProtein A column
11 Apr 2022This application note tests the recombinant protein A column at the maximum flow rate and performs the bridging study against the native protein A column. A chromatography bind/elute method for mAb titering is demonstrated, which delivers an ultrafast run time (1 minute) suitable for high-throughput applications such as clonal selection, process development, and optimization. In the bridging study, performance characteristics such as retention time, linearity and deviation of the standard curve, sample carryover, and recovery showed no detectable difference between the native and the recombinant columns.
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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.2D Chromatography