ResourceSeparations

Lysate Clearance Using the AcroPrep™ Advance Filter Plate

12 Oct 2014

Molecular biology methods have led to the successful production of a variety of synthesized biomolecules (DNA, RNA, proteins) from prokaryotic and eukaryotic cell lines. The most problematic step in the purification of these biomolecules is the clarification of the sample once the cells are lysed. This application note presents a lysate sample preparation procedure using the AcroPrep™ Advance filter plate for lysate clearance that effectively removes unwanted cellular debris from samples.

AcroPrep™ Advance Filter Plates for Lysate Clearance

Pall Life Sciences - Laboratory, Food, Beverage

The AcroPrep™ Advance filter plates for lysate clearance effectively remove cellular debris quickly and allow for the use of additional wash steps to maximize sample recovery. Filtration can be accomplished by either vacuum manifold or centrifugation, ultimately maximizing your choice in protocols. The purification of biomolecules (DNA, RNA and proteins) from prokaryotic and eukaryotic cell lines can be problematic. The AcroPrep™ Advance filters plates for lysate clearance provide a prefilter for relatively quick and easy clarification of samples: in addition, the new well geometry ensures faster flow rates and reduced hold up volumes for efficient recovery of biomolecules.Features: Integrated prefilter yields consistent filtration of samples with high levels of gross particulate New well geometry results in faster, more uniform filtration rates across the plate with reduced hold-up volume Manufactured with biologically inert materials that allow clarification of most types of lysates without loss of the target molecule Applications: Removal of Bacterial debris prior to plasmid purification Removal of bacterial and cellular debris prior to protein purification Clearance of gross particulates

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Lysate Clearance Using the AcroPrep™ Advance Filter Plate