ResourceLife Sciences
Luminescent Cell Viability and Cytotoxicity Assays on the SpectraMax i3x Multi-Mode Microplate Reader
8 Apr 2015Molecular Devices SpectraMax® i3x Multi- Mode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. This application note demonstrates that the SpectraMax i3x reader offers excellent sensitivity in luminescence detection for ATP-based cell viability and adenylate kinase-based cytotoxicity assays, enabling detection of as few as 10 cells per well.
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SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer
Molecular Devices®Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades
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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell ViabilityCell viability assays assess the health of cells and their ability to proliferate. These tests are crucial in drug development, toxicity testing, and cell-based research. Common methods for assessing viability include MTT, live/dead assays, and flow cytometry. Browse our peer-reviewed product directory to find the best tools for cell viability assays, compare products, check reviews, and get pricing directly from manufacturers.CytotoxicityCytotoxicity assays measure the toxic effects of substances on cells, often used in drug testing and environmental studies. These tests are crucial in determining the safety of chemicals and pharmaceutical compounds. Explore cytotoxicity testing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.LuminescenceLuminescence techniques detect and measure light emitted by substances during chemical reactions or biological processes. Widely used in assays for diagnostics, environmental monitoring, and drug screening, luminescence-based methods offer high sensitivity. Explore leading systems and products in our directory.