ResourceLife Sciences
LPS Activation of MAPK Signaling in Whole Blood Samples
6 Oct 2014LPS (lipopolysaccharide) is known to activate multiple signaling pathways in peripheral blood monocytes via the Toll-Like Receptor 4 (TLR4) complex. Following LPS exposure, monocytes rapidly activate all three major MAP Kinase pathways – ERK, p38, and SAP/JNK, in addition to PI3 Kinase and IKK/NFkB pathways. This application note describes a procedure for monitoring these signaling pathways in whole blood samples using flow cytometry.
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Signaling

