Low Oxygen Levels in the New Brunswick™ Galaxy® 170 R CO<sub>2</sub> Incubator Enhance the Efficiency of Reprogramming Human Somatic Cells to Pluripotency

18 Nov 2014

This application note describes a method using the Galaxy 170 R with optional O2 control to increase the efficiency of the generation of stable iPSC lines using a non-optimized and novel reagent.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.IncubatorsLaboratory incubators are used to provide a stable environment to cultivate and store samples in, giving you optimal control of temperature, humidity, oxygen and CO<sub>2</sub> levels. Incubators are necessary equipment for cell culture and microbial research. Control temperature with heated or refrigerated incubators, or the levels of CO<sub>2</sub> and oxygen with CO<sub>2</sub> incubators. Using environmental chambers, create a humid climate for testing samples. Find the best incubators in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.IpscStem Cell ResearchStem cells have the ability to produce identical daughter cells as well as progeny, which commit and differentiate. Stem cell research has exciting prospects of biomedical applications in cellular disease modeling, pharmaceutical screening, and regenerative medicine.
Low Oxygen Levels in the New Brunswick™ Galaxy® 170 R CO<sub>2</sub> Incubator Enhance the Efficiency of Reprogramming Human Somatic Cells to Pluripotency