SelectScience
Join Free
Accelerating Science
ResourceLife Sciences

Lipid Cubic Phase: Drop Setting, Condition Screening and Imaging

FORMULATRIX®, Inc.
10 May 2017

Integral membrane proteins are arguably the most important class of proteins in terms of their role in cellular interactions and their associated significance in disease pathways. Lipidic Cubic Phase (LCP) has emerged as an effective medium for crystallizing membrane proteins. The current pace of adoption of LCP-based methods has increased substantially, in spite of the steep learning curve associated with their implementation. This application note will discuss the three major steps of LCP crystallization and how automation technology is significantly facilitating the use of this powerful methodology.

Related products

Request Quote for All Products

ROCK MAKER®

FORMULATRIX®, Inc.

One Software for Your Entire Protein Crystallization Workflow One protein crystallization software to manage the entire experimentation process, from design and dispense to image viewing and analysis. ROCK MAKER® is a Laboratory Information Management System (LIMS) that is there every step of the way, with powerful yet easy-to-use tools that help you get the results you need quickly.  

(0)

ROCK IMAGER®

FORMULATRIX®, Inc.

Crystal Clear Imaging. Unparalleled Speed. ROCK IMAGER ® - Crystallization Imagers are a series of automated imaging systems designed for protein crystallization screening. Capture superior quality images of your protein drops while learning critical information about your crystals. ROCK IMAGERs are available with various plate capacities and imaging features, so there is a model to fit every lab's budget and workflow.

(5)

NT8® v4

FORMULATRIX®, Inc.

Reliable Drop Setting: Reduce Costs + Increase Reproducibility The NT8 ® - Drop Setter/Crystallization Robot is a fast, nanoliter-volume liquid handler with drop dispensing capability, including setting drops for Lipidic Cubic Phase (LCP) experiments. The 8-tip head aspirates and dispenses drops from 10 nL to 1.5 μL.

(2)

Links

FORMULATRIX®, Inc.Company website

Tags

ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein CrystallographyProtein crystallization is the process of crystallizing purified proteins for 3D structure analysis by x-ray crystallography. The main methods of protein crystallization include sitting drop, hanging drop and microbatch. It is important to control parameters such as pH, temperature and concentration. Following crystallization, detectors and software are used for data collection and analysis.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.Membrane ProteinsLipidsLipids are a diverse group of organic compounds that are insoluble in water, including fats, oils, and hormones. They are critical for cellular structure and energy storage. Explore lipid analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

Related resources

Lipid Cubic Phase: Drop Setting, Condition Screening and Imaging