ResourceSpectroscopy

Ligand Binding Mass Spectrometric Immunoassay (LB-MSIA™) Workflow with Deglycosylation for Therapeutic Antibodies

21 Aug 2016

This application note illustrates how pre-analytical deglycosylation of the antibody decreases data complexity and increases sensitivity that further improves upon the LB-MSIA work flow; a pre-clinical bio-analytical solution, based on mass spectrometric detection, specific for the bioanalysis of humanized and fully human therapeutic monoclonal antibodies using adalimumab in rodent plasma as a model biological system.

Finnpipette™ Novus Multichannel Pipettes

Thermo Fisher Scientific

Speed up repetitive dispensing into microplates. Thermo Scientific™ Finnpipette™ Novus Multichannel Pipettes feature a wide range of multichannel models for demanding laboratory tasks, including repetitive dispensing into microplates. The lightweight handle and module is designed with a balanced weight distribution preventing muscle stress and strain, and enabling longer work periods without fatigue. The light, long-lasting battery allows over 4000 pipetting actions. Features: Clear, easy to read, intuitive menu navigation New multilingual graphical user interface Backlight technology eliminates surrounding light reflections and improves contrast in low-light conditions Ten pipetting options; nine aspirate/dispense speeds Functions include forward, reverse, diluting, stepper pipetting, mixing, sequential stepping, and more Programmable for your own custom applications Easy in-lab calibration for accuracy and precision Fully autoclavable tip cone ensures sterility and avoids cross-contamination Long-life battery allows approximately 4000 pipetting operations; recharged in about one hour

(1)

Finnpipette Digital - macro range

Thermo Fisher Scientific

Today’s laboratories require a sophisticated and reliable liquid handling approach that fulfills users’ needs and allows them to cope with the ever-increasing demands in daily work. Finnpipette Digital pipettes combine ergonomic design, advanced features and uncompromising performance to even better respond to various pipetting applications in modern laboratories. Product detail: Micro pipetting for small volumes - 0.2 to 1000 µl The use of expensive reagents has made micropipetting more necessary than ever, especially in the field of biotechnology. Working in the critical range of 0.2 to 1000 µl places tough demands on pipette performance. The Finnpipette Digital meets these demands, ensuring guaranteed accuracy and precision.  Macro pipetting for large volumes - 1 ml to 10 ml Thermo has applied the expertise gained in the years of pipette development to its macro range of digital Finnpipettes (1-5 ml and 2-10 ml). The results is a lightweight, easy-to-use pipette that avoids the risks associated with glass pipettes. All macro range Finnpipettes have a unique, specially designed piston assembly which completely eliminates vortex contamination, a common problem when pipetting large volumes. With it's up-to-date streamlined design, the Finnpipette Digital offers improved ergonomics for today's exacting laboratory routines. The rounded handle, modified grippy finger rest and the large display windows enhance operator comfort and efficiency. Finnpipette Digitals also feature several breakthroughs in liquid handling technology - soft-touch tip ejection with power-boosted gearing mechanism patented and super blow-out for 0.2 - 50 µl micro volume pipetting. For absolute sterility, all Finpipette Digitals are completely autoclavable. Each pipette also has a safety label on the finger rest, and they also come with a stick-on shelf hanger for convenient storage. 

(4)

Thermo Scientific Versette

Thermo Fisher Scientific

The Thermo Scientific™ Versette is a compact automated liquid handler that offers 96- or 384-channel pipetting heads and user friendly programming options. Its reliable performance and compact size make it a flexible and convenient liquid handling solution for many kinds of laboratories. Versette is designed to perform a variety of liquid handling tasks for a wide range of applications including high-throughput immunoaffinity sample preparation with Mass Spectrometric Immunoassay (MSIA*) pipette tips. It also supports 96/384 plate replication, plate stamping and serial dilution procedures.Key Features: 96-and 384-channel pipetting heads Handle a total volume range of 0.5-300 µl All pipetting heads include RFID tags for self-identify, and utilize Thermo Scientific D.A.R.T.s tips Compact Size The six position stage uses a unique dual-level design to minimise space requirements Fits on standard benches and biological hoods User-friendly programming options: Simple pipetting procedures can be created using visual on-board graphical display For more complex protocols and advanced editing Thermo Scientific ControlMate software is available

(1)

Links

Tags

Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.GlycosylationTherapeutic AntibodiesTherapeutic antibodies are antibodies engineered for use in treating diseases. They can be designed to target specific antigens, blocking harmful interactions or modulating immune responses. Therapeutic antibodies are critical in treating conditions like cancer, autoimmune disorders, and infectious diseases. Explore the best therapeutic antibody products in our peer-reviewed product directory; compare options, check reviews, and get pricing directly from manufacturers.HRAM