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LC-MS/MS Analysis of Sphingophospholipids Using a Metal-Free Column

24 Jun 2019

In LC analyses of sphingophospholipids, phosphate groups cause significant peak tailing, and therefore loss of sensitivity and reproducibility. In this application note, YMC discusses an improved method using YMC-Triart C18 metal-free columns.

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MetabolomicsMetabolomics is the study of small metabolites (the intermediates and products of metabolism). It involves the identification and quantification of cellular metabolites using analytical technologies such as GC, HPLC, NMR, and LC/MS.
LC-MS/MS Analysis of Sphingophospholipids Using a Metal-Free Column