SelectScience
Join Free
Accelerating Science
ResourceLife Sciences

Large Scale Cultivation of CHO Cells in the Stirred Single-use Bioreactor BIOSTATR CultiBag STR

Sartorius Group
6 May 2011

In this application note, Sartorius demonstrate the performance of the BIOSTATR CultiBag STR in the batch mode cultivation of CHO in a culture volume of 200 L. Serum free media was used for the cultivation. The particular CHO clone used for this study is the in house generated DG44 ST1-C6 expressing a human IgG. Typically, it reaches cell densities between 6-7 + 106 cells/mL when grown in bioreactors in this type of media.

Related products

Request Quote for All Products

BIOSTAT® CultiBag STR Plus

Sartorius Group

Innovative Bioreactor, Sensor and Stirrer Technology The BIOSTAT® CultiBag STR Plus is a stirred single use bioreactor available from small scale to large scale cultivation up to 1000L. Its design is entirely based on the conventional gold standards coming from the stainless steel systems, allowing for easy scale-up and transfer from stainless steel solutions. The BIOSTAT® CultiBag STR Plus consists of one BIOSTAT® STR Plus Control Tower, one bag holder, a single-use CultiBag STR and superior BioPAT® MFCS/DA data logging software. Single as well as twin versions are available.It is ideal for: Cell Culture applications Industrial and academic research Process development Process optimization Pilot scale production Seed cultivation for large scale bioreactors Production scale Features: Completely single use bioreactor in traditional design Top driven stirrer with magnetic coupling Application driven fully flexible gassing system Available as single and twin version User-friendly touch screen interface for easy operation Single use optical DO & pH sensors for measurement Single use bags available with working volumes of 50L, 200L, 500L and 1000L Validation & Extractible guide available

(2)

Links

Sartorius GroupCompany website

Tags

AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :CHO

Related resources

Large Scale Cultivation of CHO Cells in the Stirred Single-use Bioreactor BIOSTATR CultiBag STR