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Label-free assay for NK cell‑mediated cytolysis

Agilent Technologies
15 Mar 2023

Natural Killer (NK) cells are bone-marrow-derived lymphocytes originally identified by their large granular morphology. In this application note, Agilent summarizes a series of experiments to demonstrate how the xCELLigence real-time cell analysis system can be applied to quantitatively, and dynamically monitor NK cell-mediated cytolysis without labeling the target cells.

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xCELLigence RTCA SP

Agilent Technologies

The Agilent xCELLigence Real-Time Cell Analysis (RTCA) SP instrument uses label-free cellular impedance to continuously monitor cell health, behavior, and function with high accuracy, sensitivity, and reproducibility. The SP model can run a 96-well electronic microplate (E-Plate 96), which is ideal for cell health characterization, immune cell-mediated killing, viral cytopathic effects, cytotoxicity, cell adhesion, cell barrier function (TEER), cell signaling (e.g., GPCR) and other related applications. The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator. Simply plate cells and begin monitoring cell behavior to obtain real-time kinetic data for assay windows that stretch from seconds to days. Online data acquisition and offline data analysis are easy to perform using RTCA Software Pro, which also supports FDA 21 CFR Part 11 compliance. For Research Use Only. Not for use in diagnostic procedures.  

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Label-free assay for NK cell‑mediated cytolysis