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KRAS Mutation Detection by Compact Sequencing Compared with Real-Time Detection PCR and Pyrosequencing in the Routine Clinical Laboratory

Anagnostics Bioanalysis
15 Nov 2013

Patients with colorectal cancer and KRAS mutations do not benefit from treatment with monoclonal antibodies to epidermal growth factor receptor (EGFR). Therefore, KRAS mutation detection has been introduced into clinical practice. Several clinical laboratory methods have been established such as direct sequencing, pyrosequencing, and real-time detection PCR, which show varying detection limits and differing grades of automation.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.Clinical TrialsClinical Trials, an essential part of drug discovery process, assess the safety and effectiveness of a new medication or device in the pharmaceutical industry. Clinical Trials are a phased process (Phase 0, Phase I, Phase II, Phase III and Phase IV) which begins after initial preclinical testing.Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.Colorectal CancerCompanion DiagnosticsCompanion diagnostics are tests, often <i>in vitro</i>, that are used to determine whether a therapeutic product will be safe and effective for use on an individual. They also identify which patients will benefit most from them. Companion diagnostic tests are often developed in conjunction with the pharmaceutical industry.

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KRAS Mutation Detection by Compact Sequencing Compared with Real-Time Detection PCR and Pyrosequencing in the Routine Clinical Laboratory