ResourceLife Sciences
Keys to Successful Cell-Based Assay Development with the MaxCyte® STX Scalable Transfection System
14 Aug 2014This application note demonstrates keys to successfully achieving optimal results with the MaxCyte STX scalable transfection system. To minimize the effects of DNA toxicity and to maximize assay performance, MaxCyte STX users should perform several small scale, static electroporations with varying concentrations of DNA in OC-100 or OC-400 processing assemblies prior to conducting a large scale, flow electroporation using a CL-2. A systematic approach to optimizing post electroporation analysis and cryopreservation conditions will allow users to maximize the capabilities of the MaxCyte STX technology and achieve the best possible assay results with their transfected cells.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Protein ExpressionProtein expression is the utilization of cell machinery for the synthesis of proteins and has become a critical tool in biotherapeutic, genomic, and proteomic research. Produce recombinant proteins with expression vectors in combination with a host cell suitable for high-level protein expression. For production of toxic proteins, consider cell-free expression vectors. Create and monitor post-translational modifications with protein modification kits. Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.TransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.ElectroporationgPCR