Isolation of DNA Fragments from Agarose Gels Using Sartobind® D Anion Exchanger

The isolation of DNA fragments from agarose gels is a standard method frequently used in molecular biology. A variety of isolation methods have been developed, all with advantages and disadvantages. This procedure describes the use of Sartobind D membranes for electrophoretic isolation of DNA fragments by insertion of small pieces of the weak anion exchange membrane into the agarose gel. It has the advantage of being inexpensive, efficient and has no contamination of agarose in the purified fragment.