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Is Retention Time Precision Only Affected by the LC Pump?
13 Mar 2015Historically, the retention time precision of a liquid chromatography (LC) system was attributed to the pump of that instrument. This poster illustrates that not only the pump is of importance but also the autosampler and detector technology. The retention time precision of a Thermo Scientific™ Vanquish™ UHPLC instrument, and the pump performance at demanding physical conditions were evaluated. The effect of a sample pre-compression and assessed the importance of sufficiently high data collection rates were also investigated.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLC Method DevelopmentIt is important to develop and validate HPLC methods in order to identify, quantify or purify compounds of interest in the most efficient way. Effective method development ensures optimization, quality control and cost effectiveness.