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IRDye® Peptide Labeling with Odyssey® Imaging Systems

LICORbio
29 Oct 2018

Peptides labeled with fluorescent dyes are important as probes for in vivo imaging and as substrates for enzyme activity assays. Near-infrared (NIR) fluorophores such as IRDye® 800CW (excitation 780 nm; emission 794 nm) can offer improved sensitivity because of low NIR autofluorescence from tissues, cells, biological materials, or drug compounds. Fluorophores can also be combined with appropriate quencher dyes to create fluorogenic peptide probes, such as those used for protease activity detection. This application note provides an overview of IRDye® Peptide Labeling with LI-COR’s Odyssey® Classic, Odyssey CLx, Odyssey Sa, and Aerius Imaging Systems.

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Near Infrared SpectroscopyNear-infrared (NIR) spectroscopy measures the interaction of near-infrared light with a sample, including transmission, reflectance and absorbance, facilitating the identification of analytes. Measurements can be conducted using a Fourier-transform near infrared (FT-NIR) spectrometer, while there are also UV-Vis- NIR spectrophotometers that measure a broader spectrum of wavelengths. Find the best NIR spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.UV-Visible SpectroscopyUltraviolet-visible (UV-Vis) spectrophotometers are used to measure the interaction of UV and visible light with a sample, including transmission, reflectance & absorbance. The two major instrument classes are single-beam or double-beam spectrophotometers. More specialized equipment includes colorimeters, spectroradiometers and refractometers. Portable and microvolume spectrophotometers are also available. For the modular spectroscopy lab, explore a range of light sources for combination with a spectrograph/spectrometer and optics. Find the best UV-Vis spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LabelingPeptidesPeptides are short chains of amino acids that play key roles in biological processes, including signaling, immune responses, and enzyme regulation. Peptide research is critical in drug discovery, diagnostics, and vaccine development. Browse our peer-reviewed product directory to find the best peptides and peptide-related products, compare options, check reviews, and get pricing directly from manufacturers.UV-Visible-NIRUV-Visible-NIR spectroscopy is used to analyze a wide range of samples based on their absorption of ultraviolet, visible, and near-infrared light. It is commonly applied in chemical analysis, material science, and quality control. Browse our peer-reviewed product directory to find the best UV-Visible-NIR spectroscopy systems, compare products, check reviews, and get pricing directly from manufacturers.

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IRDye® Peptide Labeling with Odyssey® Imaging Systems