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IP-HPLC of Risedronate

Sigma-Aldrich Supelco
8 Dec 2014

Risedronate, the active pharmaceutical ingredient in Actonel®, is a bisphosphonate drug used for the treatment and prevention of osteoporosis. In this application note, the analysis of risedronate is performed on an AscentisTM C18 column using a Fluka brand ion pair reagent. When ion pair chromatography is required, it is imperative to use the highest purity reagents available that produce clean baselines and reproducible separations.

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Ascentis® C18 HPLC column, 3micron, 15cm L x 4.6mm ID

Sigma-Aldrich Supelco

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide. The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Compound AnalysisCompound analysis is used to discover specific compounds that could be promising candidates for pharmaceutical use. This potential is identified when compounds have the desired effect, such as interaction with a target protein, during high throughput screening.Active PharmaceuticalsThe Active Pharmaceutical Ingredient (API) of a drug is the component that causes the direct effect of the product. The remaining inactive components are known as excipients.

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IP-HPLC of Risedronate