ResourceLife Sciences
Investigation of Living Cells Using JPK’s QI™ Mode
9 Nov 2012Although many adaptations and modes have been developed over the years, live cell imaging and characterization using atomic force microscopy (AFM) have remained challenging and reserved for experienced AFM specialists. This application note demonstrates how the new QI™ (Quantitative Imaging) mode from JPK makes it possible to image the most challenging of samples. QI™ mode takes a force curve at every pixel using a unique tip movement and is beneficial as living cells can be imaged without lateral forces. In addition the resulting data are much more versatile that just a few images.
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Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Live Cell ImagingLive cell imaging is the study of living cells using microscopes and high-content imaging systems. This technique provides in-depth insight into fast and complex biological processes, by allowing dynamic imaging of living cells instead of acquiring an individual image at a single point in time.