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Infrared Detection Decreases Nitrocellulose Autofluorescence and Improves RPPA Assays Signal‐to‐Noise Ratio Over Visible Wavelength Detection

Innopsys
15 Aug 2013

Reverse Phase Protein Arrays (RPPAs) are a specific type of protein arrays consisting of printed cell lysates useful for cell signaling and biomarker analysis. RPPAs are mainly built on nitrocellulose film slides as they have high binding capacity and viability for native proteins. Until now, RPPA assays with fluorescence detection were limited in sensitivity by high intrinsic background fluorescence normally produced by the nitrocellulose; in this application note learn how it is now possible to increase the Signal to Noise Ratio using Near Infrared (NIR) detection with the InnoScan 710‐IR scanner.

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Advanced Infrared 2-colour Microarray Scanner: InnoScan 710IR

Innopsys

Unmatched performance for protein assays.Specifically designed to enhance detection in nitrocellulose protein array applications, the InnoScan 710IR near infrared (NIR) fluorescence scanner from Innopsys has on-board 670 nm and 785 nm excitation lasers to optimize performance by reducing the inherent background from the nitrocellulose. No scanner comes close for optical quality and speed of operation. Full compatibility with traceability standards is guaranteed, with a validation slide and associated Mapix software provided.Eliminating one of the most common concerns, the InnoScan 710IR is insensitive to substrate distortion. The real-time autofocus system makes the entire reading area completely uniform.Sensitivity and signal-to-noise ratio are two of the outstanding characteristics upon which the reputations of InnoScan scanners have been built. Outstanding photometric properties, combined with unique data manipulation software, ensure the most advanced analyses possible on the market for this class of scanner.Features: 670 nm and 785 nm excitation lasers for enhanced IR performance Simple to understand and operate – concentrate on the interpretation High precision - maximum resolution of 3 µm/pixel Under 4 minutes reading time combined with 10µm resolution Unbeatable scanning speed (10 to 35 lines/sec) Barcode identification for reliable traceability Portable, compact design; can be shared by labs Attractive price

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Fluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.NIRSBiomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.

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