Infection of Soybean Plant Roots with GFP-Expressing Phytophthora Sojae

26 Nov 2014

A clearer understanding of the host-pathogen interaction can lead to the development of increasingly disease-resistant soybean crops or less virulent Phytophthora species. Therefore, pathogenesis study of P. sojae soybean root infection is critical and can be aided by the power of fluorescence to elucidate the molecular mechanisms of infection. This application note demonstrates that coupling the iBox Explorer2 Microscope to the study of GFP-expressing P. sojae can greatly aid in the study of its pathogenesis.

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MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Environmental Monitoring and TestingEnvironmental monitoring and testing uses handheld portable analyzers, kits, spectrometers or chromatography systems for air, water, soil, food and other sample testing. Useful features of analyzers such as BOD and COD include portability, easy calibration, automation and sensitivity.  Environmental test kits for pH, water, moisture, etc, should be accurate, sensitive, reliable, fast and easy to use.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Plant SciencePlant science studies the biology of plants, including genetics, physiology, ecology, and biotechnology. Research in this field is essential for improving crop yields, disease resistance, and sustainability. Explore plant science tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Pathogen DetectionPathogen detection is the process of identifying harmful microorganisms, including bacteria, viruses, and fungi, that can cause diseases. Accurate pathogen detection is critical for food safety, public health, and clinical diagnostics. Explore pathogen detection products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.
Infection of Soybean Plant Roots with GFP-Expressing Phytophthora Sojae