Increasing Chromatographic Productivity and Reducing Solvent Consumption with ACQUITY UPLC

22 Jan 2015

Liquid chromatography has become the mainstay of many quantitative and qualitative analyses in areas such as pharmaceutical drug analysis, environmental monitoring, food testing, and water quality monitoring. This white paper outline that the throughput, selectivity, and specificity achieved with the ACQUITY UPLC® System provides improved chromatographic results while consuming less solvent than traditional HPLC – for more efficient laboratory operations.

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ACQUITY UPC² System
Waters
Convergence Chromatography with the ACQUITY UPC² System Bring streamlined workflow, expanded selectivity, and an orthogonal technique to your laboratory. Convergence chromatography (CC) is a broad-based, complementary analytical platform that is taking its place alongside of LC and GC as one of the three essential separation technologies for modern laboratory analysis. This technique provides another, reliable choice as an analytical platform that is orthogonal to reversed-phase LC (RPLC), while significantly streamlining the entire analytical workflow. In convergence chromatography, the separation is achieved by manipulating the density and composition of a supercritical fluid-based mobile phase. Because of the very high diffusivity of the mobile phase, can be achieved. Additionally, the diversity of stationary phase and mobile phase (co-solvent) options provides access to the largest selectivity space available to any separation technique. The ACQUITY UPC² System is a holistically designed UltraPerformance Convergence Chromatography (UPC²) system that utilizes liquid CO2 as the primary mobile phase. The system leverages the chromatographic principles and selectivity of normal-phase chromatography while providing the ease-of-use of RPLC, enabling you to address routine and complex separations challenges. ACQUITY UPC² System: Discover what's been missing from your analytical toolbox With the ACQUITY UPC² System, scientists can now separate, analyze, and understand compounds that have challenged all other LC and GC technologies including: Hydrophobic and chiral compounds Lipids Thermally-labile samples Polymers It's also the perfect complement to MS due to its low solvent load and high resolution, narrow peaks and fast separations. ACQUITY UPC² System Features: Compressed carbon dioxide (CO2), the primary mobile phase, is less costly and less toxic than liquid mobile phases or carrier gases. Variable volume injection delivers 0.5 µL to 10 µL as well as the ability to match column and injection volume with minimal sample loss and need to change loops. Higher throughput and productivity allows you to analyze more samples per day with faster re-equilibration of columns and shorter cycle times, increasing laboratory productivity. Co-solvent and column switching provides flexibility in method development for faster solvent screening and faster column screening.
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ACQUITY Advanced Polymer Chromatography System
Waters
Developing differentiated polymers and deploying a sustainable operation are a key focus for the polymer and plastics industries. Current GPC/SEC methods are limited by a lack of resolution, separation times, and the volume of solvent consumed per analysis. The ACQUITY Advanced Polymer Chromatography (APC™) System is a breakthrough technology that defines the ultimate in size-based chromatographic separations, delivering more information about your polymers faster than ever before. This means better characterization, improved asset utilization and a superior solution for achieving corporate innovation and sustainability goals. Key Features: New Column Technology – ACQUITY APC™ Columns utilize small, high pore volume bridged-ethyl hybrid particles that provide significant gains in stability, flexibility and faster separations. Stable Refractive Index Detection – The ACQUITY Refractive Index (RI) Detector is optimized for low dispersion, but with the low noise and drift performance required for accurate integration, even at low polymer concentrations. Precise Solvent Management - Precise flow delivery of the isocratic solvent manager ensures calibrated system provides accurate molecular weight data, day after day. Advanced Column Heating Module - Ensures highly repeatable thermal environment for banks of ACQUITY APC Columns. Empower® 3 Software with GPC option – Quickly and easily review, compare, and report polymer analysis data. Key Benefits Polymer Characterization - Provides unparalleled polymer peak resolution, particularly for low molecular weight oligomers. Speed of Analysis - Obtain accurate and repeatable polymer molecular weight information up to 5 to 20 times faster than traditional GPC/SEC methods. Cost Reduction - Lower analysis cost by reducing solvent consumption and waste disposal volumes. System Flexibility - Run diverse polymer applications on a single system.
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ACQUITY UPLC H-Class PLUS System
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Transform standard procedures into ultimate performance.
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ACQUITY UPLC M-Class System
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The analysis you’ve always desired for nano- to microscale separations The ACQUITY UPLC M-Class System delivers the most robust, reliable, and highest quality nano- to microscale UPLC separations to every laboratory performing LC/MS analyses. With unprecedented usability, the ACQUITY UPLC M-Class System enables laboratories to succeed in analyzing compounds that are severely limited in amount or availability, even amid a complex matrix over several orders of magnitude. Confidently achieve your laboratory goals with high-efficiency ACQUITY UPLC M-Class Columns ranging from 75 µm to 1 mm I.D. by using complementary mass spectrometry to support our full range of column I.D.’s, or run 300 µm to 1 mm I.D. columns with optical detection.The system also offers a range of flexible configurations for HDX technology, UV detection, and 2D analysis.ACQUITY UPLC M-Class System Features: Highest sensitivity - UPLC separations yield increased analyte concentration for higher UV and MS signal responses Maximum chromatographic resolution - New 15k psi-capable ACQUITY UPLC M-Class Columns deliver sub-2-µm particle performance Faster nano- and microscale separations - Increased operating pressures yield maximum flexibility for higher throughput analyses Higher resolution with longer columns - Increased pressure enables more separation power Confidence in sample analysis - New non-reactive wetted surfaces throughout the fluidic path minimize absorptive losses Increased solvent compataibility - Improved range of solvent blends precisely metered for gradient fidelity Increased sample capacity - Sample organizer compatibility allows you to store a wide variety of plates and vials in the system for longer, unattended operation UV compatibility - UV flow cells compatible with 300 µm to 1.0 mm I.D. columns to augment your UPLC/MS analysis Scalability - With flow rates from 200 nl/min to 100 µL/min, achieve the best chromatographic performance, from 75 µm to 1.0 mm I.D. columns covering a 170-fold range of column sizes Increased flexiblity - More value configurations enable a variety of applications, icnreased throughput, and maximized peak capacity
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ACQUITY UPLC® I-Class PLUS System
Waters
The most powerful solution to the most critical need in separation science today.
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ACQUITY UPC² System

Waters

Convergence Chromatography with the ACQUITY UPC² System Bring streamlined workflow, expanded selectivity, and an orthogonal technique to your laboratory. Convergence chromatography (CC) is a broad-based, complementary analytical platform that is taking its place alongside of LC and GC as one of the three essential separation technologies for modern laboratory analysis. This technique provides another, reliable choice as an analytical platform that is orthogonal to reversed-phase LC (RPLC), while significantly streamlining the entire analytical workflow. In convergence chromatography, the separation is achieved by manipulating the density and composition of a supercritical fluid-based mobile phase. Because of the very high diffusivity of the mobile phase, can be achieved. Additionally, the diversity of stationary phase and mobile phase (co-solvent) options provides access to the largest selectivity space available to any separation technique. The ACQUITY UPC² System is a holistically designed UltraPerformance Convergence Chromatography (UPC²) system that utilizes liquid CO2 as the primary mobile phase. The system leverages the chromatographic principles and selectivity of normal-phase chromatography while providing the ease-of-use of RPLC, enabling you to address routine and complex separations challenges. ACQUITY UPC² System: Discover what's been missing from your analytical toolbox With the ACQUITY UPC² System, scientists can now separate, analyze, and understand compounds that have challenged all other LC and GC technologies including: Hydrophobic and chiral compounds Lipids Thermally-labile samples Polymers It's also the perfect complement to MS due to its low solvent load and high resolution, narrow peaks and fast separations. ACQUITY UPC² System Features: Compressed carbon dioxide (CO2), the primary mobile phase, is less costly and less toxic than liquid mobile phases or carrier gases. Variable volume injection delivers 0.5 µL to 10 µL as well as the ability to match column and injection volume with minimal sample loss and need to change loops. Higher throughput and productivity allows you to analyze more samples per day with faster re-equilibration of columns and shorter cycle times, increasing laboratory productivity. Co-solvent and column switching provides flexibility in method development for faster solvent screening and faster column screening.

(18)

Increasing Chromatographic Productivity and Reducing Solvent Consumption with ACQUITY UPLC

Related products

ACQUITY UPC² System

ACQUITY Advanced Polymer Chromatography System

ACQUITY UPLC H-Class PLUS System

ACQUITY UPLC M-Class System

ACQUITY UPLC® I-Class PLUS System

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ACQUITY UPC² System

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