SelectScience
Join Free
Accelerating Science
ResourceLife Sciences

Increased Efficiency of Biomolecule Identification by Optimization of Trypsin Digestion Buffers

Thermo Fisher Scientific

Increased Efficiency of Biomolecule Identification by Optimization of Trypsin Digestion Buffers

28 Oct 2015

Protein insolubility can be a major impediment to traditional bottom-up proteomic methods because it reduces the efficiency of enzymatic digestions and makes analysis problematic. Insolubility can be caused by a variety of factors, including the salt concentration, the presence of various organic solvents, temperature, or simply the nature of the protein itself. This application note demonstrates how the Thermo Scientific™ SMART Digest™ kit removes the uncertainty associated with conventional solution-based tryptic digestion protocols, resulting in higher efficiency and sample characterization.

Related products

Request Quote for All Products

Thermo Scientific™ SMART Digest™ Kits

Thermo Fisher Scientific

Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits. A significant advance in sample preparation for biopharmaceutical and protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.   Features: Easy and simple Significantly increased speed of sample processing. 3-step sample preparation. Easily automate entire protocol for high-throughput processing. Reliable results Increased reproducibility over existing protocols. Higher sensitivity. Higher data confidence.

(2)

Links

Thermo Fisher ScientificCompany website

Tags

ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Method DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.EnzymesProtein PurificationDigestion

Related resources