Increase Data Quality for Sulfonamide Residue Analysis by HPLC and UHPLC Using Unique Biphenyl Column Selectivity

31 Dec 2014

The analysis of antibiotic residues in food-producing animals is important worldwide for evaluating food safety and maintaining compliance with export regulations. Sulfonamides are a specific concern, as drugs in this antibiotic class are commonly used in feed additives for livestock in order to fight infections and maintain desired growth levels. In this application note, the unique selectivity and high retention of biphenyl columns produce complete separations and benefit both UV and MS detection. In addition, biphenyl columns in a UHPLC format allow faster sample throughput, while maintaining good separation of target compounds.

Ultra Selective Liquid Chromatography™

Restek Corp.

Reversed Phase Method Development that's Simple, Fast, Effective, and Universal!•Boasts the widest range of reversed phase selectivity in the industry.•Simplifies column selection down to 4 unique stationary phases.•Employs orthogonal selectivity to speed up effective method development.•Maximizes efficacy of your column screening systems.•Fits any HPLC or UHPLC instrumentation. Restek Ultra Selective Liquid Chromatography™ (USLC™) technology is the directed application of selectivity—the most influential factor affecting resolution—to optimize separations and improve method performance. Restek has extensively studied reversed phase selectivity, and as a result, we offer chromatographers the most effective and widest range of USLC™ stationary phase chemistries in the industry! Pinnacle® DB columns (1.9 µm) feature base deactivated silica and are recommended for scalable UHPLC and HPLC analyses. Ultra columns (3 and 5 µm) use highest purity silica and are well suited for traditional HPLC analyses. Viva columns incorporate wide pore silica, making them ideal for large-molecule biological separations using HPLC.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AntibioticsAntibiotics are substances that kill or inhibit the growth of microorganisms, such as bacteria and fungi. They are essential in the treatment of infections and in preventing disease spread. Explore antibiotics research and diagnostic tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.
Increase Data Quality for Sulfonamide Residue Analysis by HPLC and UHPLC Using Unique Biphenyl Column Selectivity