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Incorporation of Cytation™ Cell Imaging Multi-Mode Readers and Gen5™ Data Analysis Software to Create Deconvoluted, Stacked Images of 3D Cellular Structures

Agilent Technologies
7 Nov 2014

Three-dimensional (3D) cellular models have the potential to become a fundamental research tool in cell biology and histology slides containing tissue samples mounted onto microscope slides, continue to be an important research and diagnostic method in the clinic and laboratory. These multi-dimensional cell structures present complications for optical microscopy due to their thickness in the z-axis. This application note presents a method to capture and project multiple single z-plane images using digital widefield microscopy.

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Agilent BioTek Gen5 Software for Detection

Agilent Technologies

Agilent BioTek Gen5 software for Agilent BioTek multimode and single-mode microplate readers is an integrated tool for endpoint, kinetic, spectral scanning, and well area scanning. It controls all the functions of the plate reader and has powerful data analysis capabilities for a broad range of applications. Gen5 microplate reader software is also used to integrate BioTek plate readers to BioTek BioStack and other automated systems.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.SpheroidsSpheroids are clusters of cells that have been grown in 3D culture to be used as <i>in vitro</i> model systems. These 3D microtissues can be used for toxicology testing, DMPK studies and many other applications involving cell analysis.Metastasis

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Incorporation of Cytation™ Cell Imaging Multi-Mode Readers and Gen5™ Data Analysis Software to Create Deconvoluted, Stacked Images of 3D Cellular Structures