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Incorporating Molecular ID Technology with Accel-NGS<sup>®</sup> 2S MID Indexing Kits

Swift Biosciences, Inc
20 Oct 2016

This short presentation describes what molecular identifiers (MIDs) and what life science applications can benefit from MIDs. These applications include low frequency variant detection, deduplication from single read sequencing (eg. ChIP-Seq), deduplication from non-random fragmentation (eg. cell-free DNA). It also details the structure of Swift Molecular IDs, describes the bioinformatic analyses that can be carried out with them and gives important guidelines for the kits use.

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Accel-NGS® 2S MID Indexing Kits (48 reactions)

Swift Biosciences, Inc

The Accel-NGS 2S MID Indexing Kits have been designed, optimized, and validated for use withAccel-NGS 2S DNA Library Kits on Illumina platforms, and aid in low frequency variant detection, as well as accurate de-duplication of single read sequencing and sequencing from samples with non-random fragmentation. Get the most out of your NGS sequencing data with this powerful tool. Improve detection of low frequency alleles Identify unique library molecules Retain more sequencing data Designed for exome sequencing Compatible with circulating, cell-free DNA Supported DNA Sequencing Applications: Whole exome sequencing (WES) Deep targeted sequencing Cell-free DNA sequencing Single-end sequencing, such as ChIP-Seq

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Chem / BioinformaticsCheminformatics and bioinformatics are computational techniques used in chemistry and biology, respectively, for data acquisition, processing and storage. Cheminformatics focuses on compound information, whereas bioinformatics is mainly applied to analysis and modeling of genomics, genetic and sequencing information. Hardware and software is available for data acquisition, analysis, management and storage.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ChIP-SequencingBioinformaticsBioinformatics uses computational models and methodologies to study biological processes and to understand biological data.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.DNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.ASHG

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Incorporating Molecular ID Technology with Accel-NGS<sup>®</sup> 2S MID Indexing Kits