ResourceSpectroscopy
In-House Sulfur-SAD Phasing of Thaumatin from Thaumatococcus danielii
29 Oct 2018The crystal structure of Thaumatin from Thaumatococcus danielii was determined by sulfur-SAD phasing methods using data collected on a D8 VENTURE diffraction system. The D8 VENTURE features a new CMOS detector, the PHOTON 100. Using one crystal, a complete data set was collected to 1.65 Å in less than 3 hours. The experimental phases derived from the anomalous signal of the sulfur atoms allowed 95% of the protein backbone to be traced.
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X-Ray Diffraction and SpectroscopyX-Ray diffraction & spectroscopy are used in material characterization to discern the structure and elemental composition of a sample. X-Ray diffractometers (XRD) are superior instruments in elucidating the dimensional atomic structure of crystalline materials, including powders, thin films and single crystals. For large unit cells or ordered macromolecules, consider small-angle X-ray scattering (SAXS). X-ray spectroscopic techniques include X-ray fluorescence (XRF) and X-ray photoelectron spectroscopy (XPS), both providing simple and accurate methods for determining the elemental composition of a material. Energy dispersive (EDXRF) and wavelength dispersive (WDXRF) XRF spectrometers are available, as well as handheld/portable devices. High-resolution, 3D microstructure characterization of materials can be achieved with X-ray microscopes combining sub-micron resolution imaging with 3D computed tomography. Find the best XRD and XRF spectrometers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.SulfurBacteriaBacteria are microorganisms that play critical roles in the environment, human health, and biotechnology. Understanding bacterial genetics, growth, and metabolism is essential for developing antibiotics, vaccines, and improving industrial processes. Browse our peer-reviewed product directory to find the best bacterial research tools, compare products, check reviews, and get pricing directly from manufacturers.