ResourceLife Sciences
In-depth characterization of the major leukocyte cell subsets in human peripheral mononuclear cells
25 Jul 2024Flow cytometry is a powerful technique for in-depth analysis of different immune cell subpopulations of immune cells to gather a comprehensive assessment of the immune system. Spectral flow cytometry has led to advancements in our capabilities to support more markers, greater flexibility, and better data resolution. Agilent Technologies describes how a 45-color spectral flow cytometry panel was designed for the Agilent NovoCyte Opteon spectral flow cytometer to analyze the distribution of immune cells in a peripheral blood mononuclear cell (PBMC) sample.
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.
