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Impurity Analysis of Aminoglycoside Antibiotic using the Agilent InfinityLab Poroshell 120 HILIC-Z with ELSD Detection
6 Aug 2020A method is presented for analysis of aminoglycosides. The highly polar compound is seperated using HILIC, and detected using ELSD.
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Agilent 1260 Infinity III Evaporative Light Scattering Detector
Agilent TechnologiesThe 1260 Infinity III Evaporative Light Scattering Detector (ELSD), although targeted at analytical scale, can also be used with a flow splitter for preparative chromatography applications. It is highly recommended for compounds with little or no UV chromophore, since the detector technique evaporates the solvent and then measures the intensity of the scattered light, which is directly proportional to the sample concentration.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.