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Improved Throughput, Productivity, and Performance for the USP Assay for Norethindrone and Mestranol using KinetexR 2.6 µm C8 Core-Shell Columns
20 May 2012The efficiency provided by Kinetex 2.6 μm C8 core-shell columns is shown in this note to reduce analysis times and increase productivity for the USP assay for norethindrone and mestranol. Several alternatives to shorter column lengths and adjustments to flow rate were used to illustrate how the increase in efficiency can be leveraged to provide a balance between increasing productivity and reducing solvent usage.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NorethindroneMestranolUSP Method