ResourceLife Sciences
Improved Imaging of Cleared Samples with ZEISS Lightsheet Z.1: Refractive Index on Demand
16 Aug 2015This application note demonstrates a novel technique to formulate the optimum refractive index (RI) without compromising image quality or damaging the solvent-vulnerable Light Sheet Fluorescence Microscopy (LSFM) system. LSFM uses a thin plane of light (light sheet) to optically section transparent tissues or whole organisms that have been fluorescently labeled. This “RI matching” solution is based on the published 3DISCO, an organic-solvent based protocol, which requires no special equipment to provide a faster imaging solution compared to laser scanning microscopes.
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ZEISS Lightsheet Z.1 Microscope
ZEISS Research Microscopy SolutionsLight Sheet Fluorescence Microscopy for Multiview Imaging of Large Specimens.
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Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Fluorescence MicroscopyFluorescence microscopy has become an essential tool in biology, as well as in materials science. The application of many fluorochromes has made it possible to identify cells and sub-microscopic cellular components with a high degree of specificity. Using multiple fluorescence labels, different probes can simultaneously identify several target molecules.ImagingImaging techniques are essential for obtaining visual representations of samples to understand structures, processes, and function in biological, chemical, and physical research. These tools range from traditional light microscopy to advanced imaging modalities like MRI and electron microscopy, providing researchers with valuable data for diagnostics, drug discovery, and material analysis. Explore imaging solutions in our peer-reviewed product directory to compare products, check reviews, and get pricing directly from manufacturers.