ResourceSeparations
Improved Extraction and LC/MS/MS Analysis of Barbiturates from Urine Samples Using Strata™-X-Drug N SPE and Kinetex® Core-Shell HPLC/UHPLC Columns
9 Jan 2013The Strata™-X-Drug N SPE sorbent from Phenomenex is shown to provide both time and solvent savings, which can be multiplied when screening several samples at once, making it faster and more profitable for forensic labs to screen barbiturate samples. By pairing this improved extraction with a sensitive LC/MS/MS method on a Kinetex 2.6 μm C18 core-shell HPLC column, labs can resolve all 5 barbiturates screened including pentobarbital and amobarbital, which has proved difficult using older HPLC methods.
Links
Tags
UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Core-Shell TechnologyDrugs of AbuseA drug of abuse is a drug that has been taken for non-medical purposes. Such drugs can have mind-altering effects, with some causing dependence and addiction.Barbiturates