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Improve HPLC Analytics with Proper Pipetting of Difficult Liquids

16 Nov 2017

This application note demonstrates the advantages of using a positive displacement pipette, Gilson’s MICROMAN® E with capillary pistons, to pipette volatile liquids during the sample preparation for HPLC as compared to using air displacement pipettes and tips.

MICROMAN® E

Gilson, Inc.

The ergonomically designed MICROMAN® E positive displacement pipettes, along with disposable capillary piston (CP) tips, provide the highest precision when pipetting viscous, non-aqueous liquids such as oils, blood, glycerol, and more, while protecting the pipette from contamination. MICROMAN E is available in six models covering a volume range of 1 to 1000 µL. Reliable Dispensing Even with Viscous and Volatile Liquids MICROMAN E relies on positive-displacement technology to improve the precision of transferring viscous and volatile liquids. The capillary piston (CP) tips eliminate the air cushion between the sample and piston so volume accuracy is not affected by the sample’s properties. Tackle these issues with MICROMAN E: Viscous solutions—difficult to aspirate and stick to pipette tips. Volatile solutions—leakages are created from differences between solvent vapor pressure and the air pressure within the pipette. Cold liquids—delivered in excess amounts. Hot liquids—under-delivered amounts. Designed with Comfort and Performance in Mind The Red Dot Award winning design of the MICROMAN E features an improved handle shape that rests comfortably in your hand, with a large push-button for easy aspiration and dispensing. The volume-locking switch allows for easy volume adjustment and minimizes the risk of accidentally changing the volume during pipetting tasks. Overall Protection The positive-displacement technology of the MICROMAN E is ideal when dealing with infectious solutions and radiolabeled components. Since the disposable piston makes direct contact with the sample, the user and the pipette are protected. Within molecular biology and forensic sciences, any trace of a previous sample can lead to erroneous results and the disposable CP tips provide absolute protection against cross-contamination.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Pipettes and DispensersPipettes and dispensers are essential lab equipment used to dispense measured volumes of liquid accurately. Explore a range of manual and electronic pipettes, including single and multichannel, serological and volumetric pipettes, as well as a range of pipette tips. Bottle-top dispensers are also available for simple and fast solvent dispensing. Find the best pipettes & dispensers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLC Method DevelopmentIt is important to develop and validate HPLC methods in order to identify, quantify or purify compounds of interest in the most efficient way. Effective method development ensures optimization, quality control and cost effectiveness.Liquid HandlingLiquid handling involves the precise transfer, mixing, and dispensing of liquids in laboratory settings. It is essential for accurate and consistent results in biological, chemical, and pharmaceutical research. Automated liquid handling systems improve efficiency and reduce human error in sample preparation and analysis. Explore liquid handling tools in our peer-reviewed product directory; compare products, read customer reviews, and get pricing directly from manufacturers.