Imaging YFP-expressing cells with the Sapphire FL

Green fluorescent protein (GFP) and the related yellow fluorescent protein (YFP) are powerful tools frequently used as markers in a range of cellular biology experiments. The ability to detect fluorescence in living cells or organisms allows dynamic processes to be observed without affecting physiology. Azure Biosystems demonstrates the detection of YFP expressed in transformed cells using the Sapphire™ FL and 488 (YFP) Standard Optical Module. For experiments involving YFP, Azure offers a Standard Module specifically designed to detect YFP fluorescence, which includes a combination of a 488 nm laser diode and a 534/20 emission filter. This Module permits high-sensitivity detection of YFP expressed in transfected cells.