Imaging protocol handbook

25 Jun 2024

Thermo Fisher Scientific presents a trusted collection of imaging protocols, that includes step-by-step instructions for common imaging protocols, including tips for a successful experiment, and lists of needed supplies. Invitrogen™ reagents are recognized globally as key components for publication-quality cellular images and analysis. Thermo Fisher offers cellular structure and functional probes, labels, and counterstains, as well as tools for achieving stunning cellular imagery. Understand how technologies, including Invitrogen™ solutions, may be used to obtain useful cell images, such as: cell proliferation; cell viability; cell structure; live-cell assays; immunolabeling; and cell painting.

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Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 555 dye
Thermo Fisher Scientific
Alexa Fluor™ 555 dye
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Invitrogen™ Click-iT™ Plus EdU Alexa Fluor™ 350 Flow Cytometry Assay Kit
Thermo Fisher Scientific
The Click-iT Plus EdU Alexa Fluor 350 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. The Click-iT EdU Flow Cytometry Assay Kits rely on EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog which is incorporated into DNA during active DNA synthesis, which is then detected using click chemistry, a copper catalyzed covalent reaction between an azide and an alkyne. In this application, the alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to Alexa Fluor dyes. Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population. For this kit, newly synthesized DNA is analyzed using the 350 nm laser of the flow cytometer. The Click-iT Plus formulation is compatible with standard fluorophores, including R-PE and R-PE tandems, as well as fluorescent proteins.FeaturesAccurate--superior results compared to BrdU AssaysFast--results in as little as 90 minutesEconomical--more assays per kitThe Click-iT Plus EdU label is performed using much milder conditions than traditional BrdU assays. The EdU assay conditions are also compatible with cell cycle dyes and multiplexing using antibodies against surface and cellular markers.
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Invitrogen™ NucBlue™ Fixed Cell ReadyProbes™ Reagent
Thermo Fisher Scientific
High purity, room temperature stable form of DAPI
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Invitrogen™ MitoTracker™ Red FM - Special Packaging
Thermo Fisher Scientific
Far red fluorescent detection of mitochondria
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Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Red
Thermo Fisher Scientific
Room temperature-stable, ready-to-use cell viability assay
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Invitrogen™ Hoechst 33342 Ready Flow™ Reagent
Thermo Fisher Scientific
Hoechst 33342 Ready Flow Reagent is a bright, easy-to-use cell-permeable stain for cell cycle analysis on a flow cytometer. When bound to double-stranded DNA, the dye emits blue fluorescence when excited with UV light, with an emission maximum at 461 nm.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteCell-permeable and effective in fixed and live-cell staining for cell cycle
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CellEvent™ Senescence Green Detection Kit
Thermo Fisher Scientific
The CellEvent Senescence Green Detection Kit consists of a fluorescent probe and optimized buffer that enable the image-based detection of senescent cells. The fluorescein-based probe contains two galactoside moieties, making it a target for β-galactosidase. Activation of β-galactosidase is commonly used as a biomarker for senescent cells. This hydrolase enzyme resides in lysosomes and converts β-galactosides into monosaccharides under acidic pH conditions. The enzymatically clea
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Invitrogen™ BlockAid™ Blocking Solution
Thermo Fisher Scientific
High performance blocking reagent helps minimize background in cell and tissue immunofluorescence applications
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