Imaging of Single Cell Gene Expression via In Situ RT-PCR on the Mastercycler® nexus flat
23 Oct 2013In situ RT-PCR is a useful tool to detect cell types in tissue sections. Results obtained from protein expression via immunohistochemistry or quantification of mRNA by quantitative real-time RT-PCR can be checked and correlated with this method. In this application note it is demonstrated how in situ RT-PCR performance on the Mastercycler nexus flat contributes to high quality images of a single cell gene expression assay.
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Mastercycler nexus X1
EppendorfThe new Mastercycler nexus X1 combines the modern and intuitive software from the Mastercycler nexus with a fast 96-well silver block for increased heating and cooling rates. It is fast, easy to use, does not need much space or energy and sends you an e-mail when it is done—what else do you need from a PCR cycler? Combine, Connect, Control • Combine up to 3 units for maximum throughput! • Connect your Mastercycler nexus to your computer network and get a status e-mail to your desk! • Control all the relevant parameters of your PCR through the intuitive software Do you need a fast, intuitive and reliable PCR instrument? The Mastercycler nexus X1 is exactly that. It brings the reliability and ease of use of Mastercycler nexus at the speed of a silver block. Low energy consumption, easy expansion to 3 units and a small footprint make it even more attractive.