ResourceLife Sciences
Imaging of Fluorescent Microspheres Using the iBox® Explorer™ Imaging Microscope
4 Dec 2014Fluorescent microspheres are round spherical micro particles that typically range from 1 to 1000μm in diameter. They emit bright colors when illuminated by excitation sources spanning from the UV to IR regions of the spectrum. Fluorescent microspheres are loaded with a variety of proprietary dyes, making them the brightest fluorescent microspheres available. This application note demonstrates imaging of fluorescent microspheres using the iBox Explorer Imaging Microscope from UVP.
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Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Fluorescence MicroscopyFluorescence microscopy has become an essential tool in biology, as well as in materials science. The application of many fluorochromes has made it possible to identify cells and sub-microscopic cellular components with a high degree of specificity. Using multiple fluorescence labels, different probes can simultaneously identify several target molecules.CytometryCytometry is a broad term for the quantitative analysis of cells and cell systems. The most popular methods to study cytometry are flow cytometry and image cytometry.