ResourceLife Sciences
Imaging Microtissue Cores using the Opera High Content Screening System
29 Aug 2014Visualization of 3D microtissues using conventional confocal microscopy is challenging, because tissue opacity, light absorption and scattering prevent imaging at higher planes deep into the core of microtissues. This application note describes a method for detailed analysis of all cell layers within cancer microtissues using the Opera® High Content Screening System with an optical clearance solution. Pre-treatment of microtissues with the aqueous reagent Scale renders tissues optically more transparent, while fully preserving fluorescent signals in the clarified structures.
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Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.3D Imaging3D imaging technologies allow for the visualization and analysis of three-dimensional structures at high resolution. These systems are used in fields like molecular biology, material science, and medical diagnostics. 3D imaging can be applied to visualize cells, tissues, and organs, providing valuable insights into their structure and function. Browse our peer-reviewed product directory to find the best 3D imaging solutions, compare products, check reviews, and get pricing directly from manufacturers.High Content ImagingHigh content imaging is a method combining two or more fluorescent microscopy experiments to identify substances that alter a cell’s phenotype in a desired manner. The process is adapted to multi-well plates and both the image acquisition and analysis are automated.MicrotissueDrug Discovery & Development Screening